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Retroreflective optical immunosensing based on the biospecific particle movement and time-lapse imaging in microchannel
  • Lee, Kyung Won ;
  • Jeong, Kwan Young ;
  • Kim, Ka Ram ;
  • Chun, Hyeong Jin ;
  • Yoon, Hyun C.
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Publication Year
2019-01-01
Journal
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Publisher
Chemical and Biological Microsystems Society
Citation
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, pp.1246-1247
Keyword
Non-spectroscopicOptical BiosensorRetroreflective Janus ParticleWash-free Biosensor
Mesh Keyword
Cardiac troponin IHigh refractiveImmunosensingJanus particlesOptical immunosensorsOptical probeParticle movementTime-lapse imaging
All Science Classification Codes (ASJC)
BioengineeringChemical Engineering (miscellaneous)
Abstract
We developed a wash-free biosensor enabling quantitative analysis of cardiac troponin I (cTnI) using retroreflective Janus particle (RJP) as an optical probe. The RJP, we developed, had a metal layer which composed of a high refractive metal in half of the surface to induce the retroreflective phenomenon. Because of the hemisphere of the metal layer, the RJPs were sedimented and twinkled in biosensing microchannel. By using these two properties of RJP, the wash-free optical immunosensor was developed. The performance of the developed sensor was demonstrated via quantitative analysis of serum cTnI.
Language
eng
URI
https://aurora.ajou.ac.kr/handle/2018.oak/36403
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85094946976&origin=inward
Type
Conference
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