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Viscoelastic particle focusing based imaging flow cytometry: An application to yeast cells
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Publication Year
2019-01-01
Journal
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Publisher
Chemical and Biological Microsystems Society
Citation
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, pp.266-267
Keyword
Imaging flow cytometryMicrofluidicsViscoelastic particle focusing
Mesh Keyword
Flow conditionFocused-flowIndividual cellsMicro fluidic systemMultiple functionParticle focusingRectangular channelSimple geometries
All Science Classification Codes (ASJC)
BioengineeringChemical Engineering (miscellaneous)
Abstract
Flow cytometry is a tool to characterize individual cells continuously in flow. It is important to make a single stream of cells focused in the device to detect the signal from the cells efficiently. Here, we utilize the fluid viscoelasticity for 3D particle focused flow of cells. Since the fluid speed for focusing is relevantly slow, we could acquire image of moving cells in real time and could also obtain various information of the cells continuously. The viscoelastic particle focusing could be achieved in a simple geometry: straight rectangular channel, we expect that this approach can be applied to various microfluidic systems containing multiple functions under flow condition.
Language
eng
URI
https://aurora.ajou.ac.kr/handle/2018.oak/36402
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85094942020&origin=inward
Type
Conference
Funding
We are grateful to Prof. Gabor Cscus for the support of project. This research was supported by Young Researchers\u2019 Exchange Program between Korea and Switzerland (2016K1A3A1A14953247) through the National Research Foundation of Korea (NRF).
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Lee, Pyung Cheon Image
Lee, Pyung Cheon이평천
College of Bio-convergence Engineering
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