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A novel pathogen assay based on the loop-mediated isothermal amplification using retroreflective Janus particles as a nonspectroscopic signaling probe
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Publication Year
2018-01-01
Journal
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Publisher
Chemical and Biological Microsystems Society
Citation
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Vol.2, pp.788-790
Keyword
Loop-mediated isothermal amplificationMolecular diagnosticOptical biosensing systemRetroreflective Janus particlesSalmonella Typhimurium
Mesh Keyword
Janus particlesLoop mediated isothermal amplificationsMolecular diagnosticsOptical bio-sensingSalmonella typhimurium
All Science Classification Codes (ASJC)
Chemistry (all)BioengineeringChemical Engineering (miscellaneous)Control and Systems Engineering
Abstract
Loop-mediated isothermal amplification (LAMP) is a novel gene amplification method. The amplified LAMP product can be visualized via fluorescence signal or turbidity, which is generated from the fluorescent intercalating dyes or white magnesium pyrophosphate precipitate, respectively. However, these detection methods can only judge the presence or absence of target gene. Thus, quantification of target gene concentration is difficult. To overcome this limitation, we introduced a novel biosensing platform based on the LAMP assay using retroreflective Janus particle which is used as an optical probe. Using the developed sensing system, quantitative analysis of the invA in Salmonella Typhimurium are successfully accomplished.
Language
eng
URI
https://aurora.ajou.ac.kr/handle/2018.oak/36351
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85079889177&origin=inward
DOI
https://doi.org/2-s2.0-85079889177
Type
Conference
Funding
This research was mainly supported by the Samsung Research Funding Center of Samsung Electronics under the Project Number SRFC-IT1401-51. We also acknowledge the support of the National Research Foundation of Korea (NRF-2016R1A2B4006564).
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Yoon, Hyun Jin윤현진
College of Bio-convergence Engineering
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