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CORM-2-entrapped ultradeformable liposomes ameliorate acute skin inflammation in an ear edema model via effective CO deliveryoa mark
  • Lee, Gwan Yeong ;
  • Zeb, Alam ;
  • Kim, Eun Hye ;
  • Suh, Beomseon ;
  • Shin, Young Jun ;
  • Kim, Donghyun ;
  • Kim, Kyoung Won ;
  • Choe, Yeong Hwan ;
  • Choi, Ho Ik ;
  • Lee, Cheol Ho ;
  • Qureshi, Omer Salman ;
  • Han, In Bo ;
  • Chang, Sun Young ;
  • Bae, Ok Nam ;
  • Kim, Jin Ki
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Publication Year
2020-12-01
Journal
Acta Pharmaceutica Sinica B
Publisher
Chinese Academy of Medical Sciences
Citation
Acta Pharmaceutica Sinica B, Vol.10 No.12, pp.2362-2373
Keyword
Anti-inflammatory effectCarbon monoxideCORM-2Ear edemaSkin inflammationUltradeformable liposomes
All Science Classification Codes (ASJC)
Pharmacology, Toxicology and Pharmaceutics (all)
Abstract
The short release half-life of carbon monoxide (CO) is a major obstacle to the effective therapeutic use of carbon monoxide-releasing molecule-2 (CORM-2). The potential of CORM-2-entrapped ultradeformable liposomes (CORM-2-UDLs) to enhance the release half-life of CO and alleviate skin inflammation was investigated in the present study. CORM-2-UDLs were prepared by using soy phosphatidylcholine to form lipid bilayers and Tween 80 as an edge activator. The deformability of CORM-2-UDLs was measured and compared with that of conventional liposomes by passing formulations through a filter device at a constant pressure. The release profile of CO from CORM-2-UDLs was evaluated by myoglobin assay. In vitro and in vivo anti-inflammatory effects of CORM-2-UDLs were assessed in lipopolysaccharide-stimulated macrophages and TPA-induced ear edema model, respectively. The deformability of the optimized CORM-2-UDLs was 2.3 times higher than conventional liposomes. CORM-2-UDLs significantly prolonged the release half-life of CO from 30 s in a CORM-2 solution to 21.6 min. CORM-2-UDLs demonstrated in vitro anti-inflammatory activity by decreasing nitrite production and pro-inflammatory cytokine levels. Furthermore, CORM-2-UDLs successfully ameliorated skin inflammation by reducing ear edema, pathological scores, neutrophil accumulation, and inflammatory cytokines expression. The results demonstrate that CORM-2-UDLs could be used as promising therapeutics against acute skin inflammation.
ISSN
2211-3843
Language
eng
URI
https://aurora.ajou.ac.kr/handle/2018.oak/31638
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85094588327&origin=inward
DOI
https://doi.org/2-s2.0-85094588327
Journal URL
http://www.elsevier.com/wps/find/journaldescription.cws_home/725753/description#description
Type
Article
Funding
This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT ( NRF-2017R1A2B4006458 ).This work was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (NRF-2017R1A2B4006458).
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