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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 강희영 | - |
| dc.contributor.author | 김진철 | - |
| dc.date.issued | 2024-02 | - |
| dc.identifier.other | 33465 | - |
| dc.identifier.uri | https://aurora.ajou.ac.kr/handle/2018.oak/39010 | - |
| dc.description | 학위논문(박사)--의학과,2024. 2 | - |
| dc.description.tableofcontents | INTRODUCTION 1_x000D_ <br>MATERIALS AND METHODS 3_x000D_ <br> A. Cell culture 3_x000D_ <br> B. In vitro models of senescent melanocytes 3_x000D_ <br> C. Senescence associated β-galactosidase (SA-β-Gal) staining 3_x000D_ <br> D. Heterochromatin foci 3_x000D_ <br> E. Real-time PCR analysis 4_x000D_ <br> F. Western blotting 6_x000D_ <br> G. Melanin content and DOPA oxidase activity of tyrosinase 6_x000D_ <br> H. Electron microscopic observations 6_x000D_ <br> I. Immunocytochemical and immunohistochemical analysis 7_x000D_ <br> J. Single-cell transcriptomic analysis 7_x000D_ <br> K. Gene set enrichment analysis (GSEA) 8_x000D_ <br> L. Glycolytic flux analysis 8_x000D_ <br> M. Glycolysis inhibition and autophagy modulation 8_x000D_ <br> N. Measurement of ATP levels in melanocytes 9_x000D_ <br> O. Statistical analysis 9_x000D_ <br>RESULTS 10_x000D_ <br> A. Chronic UVB-exposure induces melanocyte senescence 10_x000D_ <br> B. Senescent melanocytes exhibit increased melanin contents without any increase of melanogenesis 16_x000D_ <br> C. Melanosome transport process and autophagy function are impaired in senescent melanocytes 23_x000D_ <br> D. Autophagy modulation exerts diverse effects on cellular senescence states in UVSM 31_x000D_ <br> E. Single-cell transcriptomics reveal glycolytic metabolism dysfunction in UVSM 38_x000D_ <br> F. Regulation of glycolytic metabolism alleviates senescence in melanocytes 46_x000D_ <br>DISCUSSION 54_x000D_ <br>CONCLUSION 61_x000D_ <br>REFERENCES 62_x000D_ <br>국문요약 67 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Research on the biomolecular characteristics and mechanisms of senescent melanocytes | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 대학원 | - |
| dc.contributor.alternativeName | Jin Cheol Kim | - |
| dc.contributor.department | 일반대학원 의학과 | - |
| dc.date.awarded | 2024-02 | - |
| dc.description.degree | Doctor | - |
| dc.identifier.url | https://dcoll.ajou.ac.kr/dcollection/common/orgView/000000033465 | - |
| dc.subject.keyword | autophagy | - |
| dc.subject.keyword | melanocyte | - |
| dc.subject.keyword | melanosome | - |
| dc.subject.keyword | photoaging | - |
| dc.subject.keyword | senescence | - |
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