강희영 김진철 2024-02 33465 https://aurora.ajou.ac.kr/handle/2018.oak/39010 학위논문(박사)--의학과,2024. 2 INTRODUCTION 1_x000D_ <br>MATERIALS AND METHODS 3_x000D_ <br> A. Cell culture 3_x000D_ <br> B. In vitro models of senescent melanocytes 3_x000D_ <br> C. Senescence associated β-galactosidase (SA-β-Gal) staining 3_x000D_ <br> D. Heterochromatin foci 3_x000D_ <br> E. Real-time PCR analysis 4_x000D_ <br> F. Western blotting 6_x000D_ <br> G. Melanin content and DOPA oxidase activity of tyrosinase 6_x000D_ <br> H. Electron microscopic observations 6_x000D_ <br> I. Immunocytochemical and immunohistochemical analysis 7_x000D_ <br> J. Single-cell transcriptomic analysis 7_x000D_ <br> K. Gene set enrichment analysis (GSEA) 8_x000D_ <br> L. Glycolytic flux analysis 8_x000D_ <br> M. Glycolysis inhibition and autophagy modulation 8_x000D_ <br> N. Measurement of ATP levels in melanocytes 9_x000D_ <br> O. Statistical analysis 9_x000D_ <br>RESULTS 10_x000D_ <br> A. Chronic UVB-exposure induces melanocyte senescence 10_x000D_ <br> B. Senescent melanocytes exhibit increased melanin contents without any increase of melanogenesis 16_x000D_ <br> C. Melanosome transport process and autophagy function are impaired in senescent melanocytes 23_x000D_ <br> D. Autophagy modulation exerts diverse effects on cellular senescence states in UVSM 31_x000D_ <br> E. Single-cell transcriptomics reveal glycolytic metabolism dysfunction in UVSM 38_x000D_ <br> F. Regulation of glycolytic metabolism alleviates senescence in melanocytes 46_x000D_ <br>DISCUSSION 54_x000D_ <br>CONCLUSION 61_x000D_ <br>REFERENCES 62_x000D_ <br>국문요약 67 eng The Graduate School, Ajou University 아주대학교 논문은 저작권에 의해 보호받습니다. Research on the biomolecular characteristics and mechanisms of senescent melanocytes Thesis 아주대학교 대학원 Jin Cheol Kim 일반대학원 의학과 2024-02 Doctor https://dcoll.ajou.ac.kr/dcollection/common/orgView/000000033465 autophagy melanocyte melanosome photoaging senescence