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CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells
  • Sergeeva, Daria ;
  • Camacho-Zaragoza, Jose Manuel ;
  • Lee, Jae Seong ;
  • Kildegaard, Helene Faustrup
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Publication Year
2019-01-01
Journal
Methods in Molecular Biology
Publisher
Humana Press Inc.
Citation
Methods in Molecular Biology, Vol.1961, pp.213-232
Keyword
Chinese hamster ovary cellsCRISPR/Cas9Genome editingTargeted integration
Mesh Keyword
AnimalsCHO CellsCricetinaeCricetulusCRISPR-Cas SystemsGene EditingGenome
All Science Classification Codes (ASJC)
Molecular BiologyGenetics
Abstract
The emergence of CRISPR/Cas9 system as a precise and affordable method for genome editing has prompted its rapid adoption for the targeted integration of transgenes in Chinese hamster ovary (CHO) cells. Targeted gene integration allows the generation of stable cell lines with a controlled and predictable behavior, which is an important feature for the rational design of cell factories aimed at the large-scale production of recombinant proteins. Here we present the protocol for CRISPR/Cas9-mediated integration of a gene expression cassette into a specific genomic locus in CHO cells using homology-directed DNA repair.
Language
eng
URI
https://aurora.ajou.ac.kr/handle/2018.oak/37042
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85063713049&origin=inward
DOI
https://doi.org/2-s2.0-85063713049
Journal URL
http://www.springer.com/series/7651
Type
Book Chapter
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College of Bio-convergence Engineering
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