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KSCBi005-A-10(hiPSC-HIF1αKO), a HIF1α knockout human induced pluripotent stem cell line, for demonstrating the role of cellular response to hypoxiaoa mark
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Publication Year
2024-06-01
Journal
Stem Cell Research
Publisher
Elsevier B.V.
Citation
Stem Cell Research, Vol.77
Mesh Keyword
AnimalsCell DifferentiationCell HypoxiaCell LineCRISPR-Cas SystemsGene EditingGene Knockout TechniquesHumansHypoxia-Inducible Factor 1, alpha SubunitInduced Pluripotent Stem Cells
All Science Classification Codes (ASJC)
Developmental BiologyCell Biology
Abstract
Under hypoxia, hypoxia-inducible factor (HIF)-1 regulates hypoxia-inducible genes, such as vascular endothelial growth factor (VEGF) and its receptors VEGFR1 and VEGFR2. It is an oxygen-dependent transcriptional activator that plays a crucial role in tumor angiogenesis and mammalian embryo development. It is a heterodimeric protein comprising a constitutively expressed HIF-1β subunit and the highly regulated HIF-1α subunits. Using CRISPR-Cas9 genome editing, we generated biallelic HIF-1α mutants in human induced pluripotent stem cells (hiPSCs). The HIF-1α homozygous-knockout hiPSCs retained their normal morphology, gene expression, and in vivo differentiation potential.
ISSN
1876-7753
Language
eng
URI
https://aurora.ajou.ac.kr/handle/2018.oak/34131
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85190453069&origin=inward
DOI
https://doi.org/10.1016/j.scr.2024.103415
Journal URL
https://www.sciencedirect.com/science/journal/18735061
Type
Article
Funding
This study was supported by the KCDC ( 2022-NG-009-00 ).This study was supported by the KCDC (2022-NG-009-00) and MFDS (21153MFDS602).
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Kim, Jung-Hyun김정현
Division of Pharmacy Sciences
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