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Engineering of CYP153A33 With Enhanced Ratio of Hydroxylation to Overoxidation Activity in Whole-Cell Biotransformation of Medium-Chain 1-Alkanolsoa mark
  • Park, Hyuna ;
  • Bak, Doyeong ;
  • Jeon, Wooyoung ;
  • Jang, Minjung ;
  • Ahn, Jung Oh ;
  • Choi, Kwon Young
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Publication Year
2022-01-03
Journal
Frontiers in Bioengineering and Biotechnology
Publisher
Frontiers Media S.A.
Citation
Frontiers in Bioengineering and Biotechnology, Vol.9
Keyword
CYP153A33enzyme engineeringover-oxidationwhole-cell biotransformationα,ω-alkanediol
Mesh Keyword
1-Alkanols1-dodecanolAdhesive reagentsAlkanediolsCyp153a33Dodecanoic acidEnzyme engineeringOveroxidationsWhole-cell biotransformationsΑ,ω-alkanediol
All Science Classification Codes (ASJC)
BiotechnologyBioengineeringHistologyBiomedical Engineering
Abstract
α,ω-Dodecanediol is a versatile material that has been widely used not only as an adhesive and crosslinking reagent, but also as a building block in the pharmaceutical and polymer industries. The biosynthesis of α,ω-dodecanediol from fatty derivatives, such as dodecane and dodecanol, requires an ω-specific hydroxylation step using monooxygenase enzymes. An issue with the whole-cell biotransformation of 1-dodecanol using cytochrome P450 monooxygenase (CYP) with ω-specific hydroxylation activity was the low conversion and production of the over-oxidized product of dodecanoic acid. In this study, CYP153A33 from Marinobacter aquaeolei was engineered to obtain higher ω-specific hydroxylation activity through site-directed mutagenesis. The target residue was mutated to increase flux toward α,ω-dodecanediol synthesis, while reducing the generation of the overoxidation product of dodecanoic acid and α,ω-dodecanedioic acid. Among the evaluated variants, CYP153A33 P136A showed a significant increase in 1-dodecanol conversion, i.e., 71.2% (7.12 mM from 10 mM 1-dodecanol), with an increased hydroxylation to over-oxidation activity ratio, i.e., 32.4. Finally, the applicability of this engineered enzyme for ω-specific hydroxylation against several 1-alkanols, i.e., from C6 to C16, was investigated and discussed based on the structure-activity relationship.
ISSN
2296-4185
Language
eng
URI
https://aurora.ajou.ac.kr/handle/2018.oak/32490
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85123047061&origin=inward
DOI
https://doi.org/2-s2.0-85123047061
Journal URL
http://journal.frontiersin.org/journal/bioengineering-and-biotechnology#archive
Type
Article
Funding
This study was performed with the support of the R&D Program of MOTIE/KEIT (grant number 20014350 and 20002734).
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College of Bio-convergence Engineering
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