Ajou University repository

Identification of peptide inhibitors of matrix metalloproteinase 1 using an in-house assay system for the enzyme
  • Min, Moon Won ;
  • Kim, Chae Eun ;
  • Chauhan, Sushma ;
  • Park, Hyeon Ji ;
  • Park, Chang Seo ;
  • Yoo, Tae Hyeon ;
  • Kang, Taek Jin
Citations

SCOPUS

0

Citation Export

Publication Year
2019-08-01
Journal
Enzyme and Microbial Technology
Publisher
Elsevier Inc.
Citation
Enzyme and Microbial Technology, Vol.127, pp.65-69
Keyword
Collagen degradationMatrix metalloproteinase 1Peptide inhibitorProtease inhibitionZymogen
Mesh Keyword
Collagen degradationsMatrix metalloproteinase-1Peptide inhibitorsProtease inhibitionZymogenDrug Evaluation, PreclinicalMatrix Metalloproteinase 1Matrix Metalloproteinase InhibitorsPeptides
All Science Classification Codes (ASJC)
BiotechnologyBioengineeringBiochemistryApplied Microbiology and Biotechnology
Abstract
Matrix metalloproteinases (MMPs) are zinc-dependent proteases involved in the degradation of extracellular matrix proteins. As one of the isoforms, MMP-1 breaks down collagen, and its activity is known to be important in wound healing. Its timely and adequate level of expression is pivotal because MMP-1 is also involved in the damage or aging of skins as well as in certain types of cancers. Thus, both assaying the MMP-1 activity and developing its inhibitors are of great importance. We here developed an in-house assay system that gave us the high degree of freedom in screening peptide inhibitors of MMP-1. The assay system utilized a circularly permutated fusion of β-lactamase and its inhibitory protein through an MMP-1-sensitive linker so that the activity of MMP-1 could be translated into that of β-lactamase. As a proof of concept, we applied the developed assay system to initial screens of MMP-1 inhibitors and successfully identified one lead peptide that inhibited the collagenase activity of the enzyme.
ISSN
1879-0909
Language
eng
URI
https://aurora.ajou.ac.kr/handle/2018.oak/30686
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064659591&origin=inward
DOI
https://doi.org/2-s2.0-85064659591
Journal URL
www.elsevier.com/locate/enzmictec
Type
Article
Funding
This work was supported by research grants from Ministry of Trade, Industry and Energy through the Korean Evaluation Institute of Industrial Technology ( 20002810 ) and the Ministry of Health & Welfare of Korea ( HN10C0000 ).
Show full item record

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Yoo, Tae Hyeon Image
Yoo, Tae Hyeon유태현
College of Bio-convergence Engineering
Read More

Total Views & Downloads

File Download

  • There are no files associated with this item.