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A New Infrared Probe Targeting Mitochondria via Regulation of Molecular Hydrophobicityoa mark
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Publication Year
2019-01-16
Journal
Bioconjugate Chemistry
Publisher
American Chemical Society
Citation
Bioconjugate Chemistry, Vol.30 No.1, pp.210-217
Mesh Keyword
Fluorescent coresFluorescent probesFluorochromesInfrared probesMitochondriasMolecular hydrophobicityNear InfraredNear-infraredNear-infrared fluorescentRhodamineAnimalsCell LineCell Line, TumorFluorescent DyesHeLa CellsHumansHydrophobic and Hydrophilic InteractionsMiceMitochondriaOptical ImagingRAW 264.7 CellsRhodamines
All Science Classification Codes (ASJC)
BiotechnologyBioengineeringBiomedical EngineeringPharmacologyPharmaceutical ScienceOrganic Chemistry
Abstract
Herein, we developed a near-infrared (NIR) fluorescent probe for mitochondrial staining based on the NIR fluorochrome, silicon-rhodamine. The hydrophobicity of the fluorescent core was systematically modified by conjugation with 10 different commercial amines. The resulting fluorescent compounds exhibited similar photophysical properties but diverse hydrophobicity. We identified the optimal level of hydrophobicity associated with high mitochondrial targeting efficiency. In particular, the SiR-Mito 8 probe provided excellent mitochondrial staining and successfully differentiated the live Hep3B cancer cells from normal L02 cells in vitro.
ISSN
1520-4812
Language
eng
URI
https://aurora.ajou.ac.kr/handle/2018.oak/30541
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85059742010&origin=inward
DOI
https://doi.org/10.1021/acs.bioconjchem.8b00845
Journal URL
http://pubs.acs.org/journal/bcches
Type
Article
Funding
This work was supported by Basic Research Program (2009-0093826, 2016R1E1A1A01941213 and 2016R1C1B2014699) through the National Research Foundation of Korea (NRF) funded by the Korean Government (Ministry of Science and ICT) and by the Ajou University research fund.
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Kim, Jong Hyun김종현
Department of Applied Chemistry & Biological Engineering
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