A cyclocyanine (CC)-based organic small molecule two-photon (TP) fluorescent probe (CCNa1) was developed for mitochondrial sodium ion sensing.CCNa1exhibits a low solvatochromic shift and strong TP fluorescence enhancement at 575 nm upon binding to Na+and is insensitive to other metal ions and to pH.CCNa1demonstrated fast cell loading ability, biocompatibility, and sensitive response to mitochondrial Na+influx in live cells and mouse brain tissue.
This study was supported by grants from the Advanced Technology Center (ATC) Program (10076988) funded by the Ministry of Trade, Industry & Energy, and the National Leading Research Lab Program of the National Research Foundation of Korea (NRF) (NRF-2019R1A2B5B03100278), and Center for Convergence Research of Neurological Disorders (NRF-2019R1A5A2026045).
