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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Daechan Park | - |
| dc.contributor.author | 서진아 | - |
| dc.date.issued | 2024-02 | - |
| dc.identifier.other | 33787 | - |
| dc.identifier.uri | https://aurora.ajou.ac.kr/handle/2018.oak/39130 | - |
| dc.description | 학위논문(석사)--분자과학기술학과,2024. 2 | - |
| dc.description.tableofcontents | I. Introduction 1_x000D_ <br>II. Materials and Methods 4_x000D_ <br> A. 3’ end targeted amplification by ligation-free method 4_x000D_ <br> B. 3’ end targeted amplification by direct ligation method 8_x000D_ <br> C. Preparation of spike-ins 8_x000D_ <br> D. Next-generation sequencing library construction 11_x000D_ <br> E. Process of read filtering 14_x000D_ <br> F. The algorithm strategy of poly(A) tail length measurement in 3AIM-seq 14_x000D_ <br> G. The method of poly(A) tail length measurement based on base calling 15_x000D_ <br>III. Results 16_x000D_ <br> A. Base quality scores indicated the poly(A) length 16_x000D_ <br> B. Strategies for optimal poly(A) targeted sequencing: experimental and analytic approaches 19_x000D_ <br> C. NGS library preparation from poly(A) targeted amplicons 23_x000D_ <br> D. Ligation-free method was more consistent and efficient than direct ligation method 28_x000D_ <br> E. 3AIM-seq estimates poly(A) tail length with high accuracy and resolution 38_x000D_ <br> F. IVT mRNA with long poly(A) included truncated poly(A) 49_x000D_ <br>IV. Discussion 58_x000D_ <br>V. Reference 61 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 3AIM-seq | - |
| dc.title.alternative | 3AIM-seq: 3' 말단 타겟 시퀀싱을 통한 mRNA 치료제의 폴리(A) 꼬리 길이 평가 | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 대학원 | - |
| dc.contributor.alternativeName | Jina Seo | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2024-02 | - |
| dc.description.degree | Master | - |
| dc.identifier.url | https://dcoll.ajou.ac.kr/dcollection/common/orgView/000000033787 | - |
| dc.subject.keyword | Bioinformatics | - |
| dc.subject.keyword | In vitro transcribed mRNA | - |
| dc.subject.keyword | mRNA therapeutics | - |
| dc.subject.keyword | poly(A) | - |
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