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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 진효언 | - |
| dc.contributor.author | 박하율 | - |
| dc.date.issued | 2024-08 | - |
| dc.identifier.other | 34190 | - |
| dc.identifier.uri | https://aurora.ajou.ac.kr/handle/2018.oak/39097 | - |
| dc.description | 학위논문(석사)--약학과,2024. 8 | - |
| dc.description.tableofcontents | 1. Introduction 1_x000D_ <br>2. Materials and Methods 5 _x000D_ <br> 2.1 Materials 5_x000D_ <br> 2.2 Nectin-4 expression in human cancer and normal tissue datasets 6_x000D_ <br> 2.3 Mouse immunization 6_x000D_ <br> 2.4 Mouse serum ELISA 6_x000D_ <br> 2.5 Cell fusion 7_x000D_ <br> 2.6 ELISA of hybridoma-cultured supernatants 9_x000D_ <br> 2.7 Hybridoma sequencing 10_x000D_ <br> 2.8 Hybridoma cloning 11_x000D_ <br> 2.9 Transfection of the cloned vector into mammalian cells 12_x000D_ <br> 2.10 Antibody purification 12_x000D_ <br> 2.11 Western blot 13_x000D_ <br>3. Results 15_x000D_ <br> 3.1 Analysis of mRNA data of nectin-4 in human cancers and normal tissues 15_x000D_ <br> 3.2 The expression levels of nectin-4 in ovarian cancer cells 15_x000D_ <br> 3.3 Preparation of hybridoma 16_x000D_ <br> 3.4 Screening of hybridoma subclones producing anti-nectin-4 mAbs 16_x000D_ <br> 3.5 The amino acid sequence analysis of variable domains 17_x000D_ <br> 3.6 Cloning variable domains into a single mammalian expression vector 17_x000D_ <br> 3.7 Expression and purification of recombinant anti-nectin-4 mAbs 18_x000D_ <br>4. Discussion 19_x000D_ <br>5. Conclusion 22_x000D_ <br>References 32_x000D_ <br>-국문요약- 36 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Engineering a Recombinant Monoclonal Antibody Targeting Nectin-4 via Hybridoma Technology for Ovarian Cancer Therapy | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 대학원 | - |
| dc.contributor.alternativeName | Ha-Yul Park | - |
| dc.contributor.department | 일반대학원 약학과 | - |
| dc.date.awarded | 2024-08 | - |
| dc.description.degree | Master | - |
| dc.identifier.url | https://dcoll.ajou.ac.kr/dcollection/common/orgView/000000034190 | - |
| dc.subject.keyword | Hybridoma cloning | - |
| dc.subject.keyword | Hybridoma technology | - |
| dc.subject.keyword | Nectin cell adhesion molecule 4 | - |
| dc.subject.keyword | Ovarian cancer | - |
| dc.subject.keyword | Recombinant monoclonal antibodies | - |
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