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Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells
  • Grav, Lise Marie ;
  • Rojek, Johan Blatt ;
  • la Cour Karottki, Karen Julie ;
  • Lee, Jae Seong ;
  • Kildegaard, Helene Faustrup
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dc.contributor.authorGrav, Lise Marie-
dc.contributor.authorRojek, Johan Blatt-
dc.contributor.authorla Cour Karottki, Karen Julie-
dc.contributor.authorLee, Jae Seong-
dc.contributor.authorKildegaard, Helene Faustrup-
dc.date.issued2025-01-01-
dc.identifier.urihttps://aurora.ajou.ac.kr/handle/2018.oak/37045-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85207857320&origin=inward-
dc.description.abstractGenome editing has become an important aspect of Chinese hamster ovary (CHO) cell line engineering for improving the production of recombinant protein therapeutics. Currently, the engineering focus is directed toward expanding product diversity while controlling and improving product quality and yields. In this chapter, we present our protocol for using the genome editing tool Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) to knock out engineering target genes in CHO cells. As an example, we describe how to knock out the glutamine synthetase (GS) gene, which increases the selection efficiency of the GS-mediated gene amplification system.-
dc.description.sponsorshipWe thank Karen Kathrine Br\\u00F8ndum and Johnny Arnsdorf for optimizing and setting up FACS sorting and Nachon Charanyanonda Petersen for help with the transfection and FACS sorting. This work was supported by the Novo Nordisk Foundation.-
dc.language.isoeng-
dc.publisherHumana Press Inc.-
dc.subject.meshAnimals-
dc.subject.meshCHO Cells-
dc.subject.meshCricetulus-
dc.subject.meshCRISPR-Cas Systems-
dc.subject.meshGene Editing-
dc.subject.meshGene Knockout Techniques-
dc.subject.meshGlutamate-Ammonia Ligase-
dc.subject.meshRecombinant Proteins-
dc.subject.meshRNA, Guide, CRISPR-Cas Systems-
dc.titleApplication of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells-
dc.typeBook Chapter-
dc.citation.endPage69-
dc.citation.startPage49-
dc.citation.titleMethods in Molecular Biology-
dc.citation.volume2853-
dc.identifier.bibliographicCitationMethods in Molecular Biology, Vol.2853, pp.49-69-
dc.identifier.doi10.1007/978-1-0716-4104-0_5-
dc.identifier.pmid39460914-
dc.identifier.scopusid2-s2.0-85207857320-
dc.identifier.urlhttps://www.springer.com/series/7651-
dc.subject.keywordCas9-
dc.subject.keywordChinese hamster ovary (CHO) cells-
dc.subject.keywordCRISPR-
dc.subject.keywordGenome editing-
dc.subject.keywordGlutamine synthetase-
dc.subject.keywordKnockout-
dc.subject.keywordRecombinant protein production-
dc.type.otherBook Chapter-
dc.description.isoafalse-
dc.subject.subareaMolecular Biology-
dc.subject.subareaGenetics-
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