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DC Field | Value | Language |
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dc.contributor.author | Kim, Chuna | - |
dc.contributor.author | Sung, Sanghyun | - |
dc.contributor.author | Kim, Jong Seo | - |
dc.contributor.author | Lee, Hyunji | - |
dc.contributor.author | Jung, Yoonseok | - |
dc.contributor.author | Shin, Sanghee | - |
dc.contributor.author | Kim, Eunkyeong | - |
dc.contributor.author | Seo, Jenny J. | - |
dc.contributor.author | Kim, Jun | - |
dc.contributor.author | Kim, Daeun | - |
dc.contributor.author | Niida, Hiroyuki | - |
dc.contributor.author | Kim, V. Narry | - |
dc.contributor.author | Park, Daechan | - |
dc.contributor.author | Lee, Junho | - |
dc.date.issued | 2021-12-01 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://aurora.ajou.ac.kr/handle/2018.oak/31851 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85100884760&origin=inward | - |
dc.description.abstract | Telomeres are part of a highly refined system for maintaining the stability of linear chromosomes. Most telomeres rely on simple repetitive sequences and telomerase enzymes to protect chromosomal ends; however, in some species or telomerase-defective situations, an alternative lengthening of telomeres (ALT) mechanism is used. ALT mainly utilises recombination-based replication mechanisms and the constituents of ALT-based telomeres vary depending on models. Here we show that mouse telomeres can exploit non-telomeric, unique sequences in addition to telomeric repeats. We establish that a specific subtelomeric element, the mouse template for ALT (mTALT), is used for repairing telomeric DNA damage as well as for composing portions of telomeres in ALT-dependent mouse embryonic stem cells. Epigenomic and proteomic analyses before and after ALT activation reveal a high level of non-coding mTALT transcripts despite the heterochromatic nature of mTALT-based telomeres. After ALT activation, the increased HMGN1, a non-histone chromosomal protein, contributes to the maintenance of telomere stability by regulating telomeric transcription. These findings provide a molecular basis to study the evolution of new structures in telomeres. | - |
dc.description.sponsorship | The authors thank Michael Bustin for sharing HMGN1 antibody. Long read Iso-Seq was provided by the SMRT Grant of MDxK. This work was supported by the National Research Foundation of Korea (NRF-2020R1A2C3003352, NRF-2019R1C1C1008181, and NRF-2020R1C1C101220611) and the Institute for Basic Science (IBS-R008-D1). C.K. was supported by the KRIBB Research Initiative Programme. The authors thank Daisy Sunghee Lim for creating model images 1a, 3d, and 6. | - |
dc.language.iso | eng | - |
dc.publisher | Nature Research | - |
dc.subject.mesh | Animals | - |
dc.subject.mesh | DNA-Binding Proteins | - |
dc.subject.mesh | Epigenomics | - |
dc.subject.mesh | HEK293 Cells | - |
dc.subject.mesh | Humans | - |
dc.subject.mesh | Mice | - |
dc.subject.mesh | Mice, 129 Strain | - |
dc.subject.mesh | Mice, Inbred C57BL | - |
dc.subject.mesh | Mouse Embryonic Stem Cells | - |
dc.subject.mesh | Proteomics | - |
dc.subject.mesh | Repetitive Sequences, Nucleic Acid | - |
dc.subject.mesh | Sequence Analysis, RNA | - |
dc.subject.mesh | Single-Cell Analysis | - |
dc.subject.mesh | Telomerase | - |
dc.subject.mesh | Telomere | - |
dc.subject.mesh | Telomere Homeostasis | - |
dc.subject.mesh | Transcription Factors | - |
dc.title | Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells | - |
dc.type | Article | - |
dc.citation.number | 1 | - |
dc.citation.title | Nature Communications | - |
dc.citation.volume | 12 | - |
dc.identifier.bibliographicCitation | Nature Communications, Vol.12 No.1 | - |
dc.identifier.doi | 2-s2.0-85100884760 | - |
dc.identifier.pmid | 33597549 | - |
dc.identifier.scopusid | 2-s2.0-85100884760 | - |
dc.identifier.url | http://www.nature.com/ncomms/index.html | - |
dc.type.other | Article | - |
dc.description.isoa | true | - |
dc.subject.subarea | Chemistry (all) | - |
dc.subject.subarea | Biochemistry, Genetics and Molecular Biology (all) | - |
dc.subject.subarea | Physics and Astronomy (all) | - |
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