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Mitigating Clonal Variation in Recombinant Mammalian Cell Lines
  • Lee, Jae Seong ;
  • Kildegaard, Helene Faustrup ;
  • Lewis, Nathan E. ;
  • Lee, Gyun Min
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dc.contributor.authorLee, Jae Seong-
dc.contributor.authorKildegaard, Helene Faustrup-
dc.contributor.authorLewis, Nathan E.-
dc.contributor.authorLee, Gyun Min-
dc.date.issued2019-09-01-
dc.identifier.issn1879-3096-
dc.identifier.urihttps://aurora.ajou.ac.kr/handle/2018.oak/30645-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85063035419&origin=inward-
dc.description.abstractMammalian expression platforms are primary production systems for therapeutic proteins that require complex post-translational modifications. Current processes used for developing recombinant mammalian cell lines generate clonal cell lines with high phenotypic heterogeneity, which has puzzled researchers that use mammalian cell culture systems for a long time. Advances in mammalian genome-editing technologies and systems biotechnology have shed light on clonal variation and enabled rational cell engineering in a targeted manner. We propose a new approach for a next-generation cell line development platform that can minimize clonal variation. Combined with the knowledge-based selection of ideal integration sites and engineering targets, targeted integration-based cell line development will allow tailored control of recombinant gene expression with predicted phenotypes.-
dc.description.sponsorshipThis work was supported in part by the Novo Nordisk Foundation ( NNF10CC1016517 ) and the NRF funded by the Korean government ( 2018R1C1B6001423 ). The authors declare no competing financial interests.-
dc.description.sponsorshipThis work was supported in part by the Novo Nordisk Foundation (NNF10CC1016517) and the NRF funded by the Korean government (2018R1C1B6001423). The authors declare no competing financial interests.-
dc.language.isoeng-
dc.publisherElsevier Ltd-
dc.subject.meshCell lines-
dc.subject.meshClonal variations-
dc.subject.meshDevelopment platform-
dc.subject.meshMammalian cell culture-
dc.subject.meshMammalian cell lines-
dc.subject.meshPost-translational modifications-
dc.subject.meshRecombinant gene expressions-
dc.subject.meshTransgene-
dc.subject.meshAnimals-
dc.subject.meshCell Engineering-
dc.subject.meshCell Line-
dc.subject.meshGene Editing-
dc.subject.meshGenetic Engineering-
dc.subject.meshMammals-
dc.subject.meshRecombinant Proteins-
dc.titleMitigating Clonal Variation in Recombinant Mammalian Cell Lines-
dc.typeArticle-
dc.citation.endPage942-
dc.citation.number9-
dc.citation.startPage931-
dc.citation.titleTrends in Biotechnology-
dc.citation.volume37-
dc.identifier.bibliographicCitationTrends in Biotechnology, Vol.37 No.9, pp.931-942-
dc.identifier.doi10.1016/j.tibtech.2019.02.007-
dc.identifier.pmid30898338-
dc.identifier.scopusid2-s2.0-85063035419-
dc.identifier.urlwww.elsevier.com/locate/tibtech-
dc.subject.keywordcell line development-
dc.subject.keywordclonal variation-
dc.subject.keywordrational cell engineering-
dc.subject.keywordtransgene integration-
dc.type.otherReview-
dc.identifier.pissn0167-7799-
dc.description.isoafalse-
dc.subject.subareaBiotechnology-
dc.subject.subareaBioengineering-
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