Citation Export
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sudheer, Pamidimarri D.V.N. | - |
dc.contributor.author | Seo, Dahee | - |
dc.contributor.author | Kim, Eun Joo | - |
dc.contributor.author | Chauhan, Sushma | - |
dc.contributor.author | Chunawala, J. R. | - |
dc.contributor.author | Choi, Kwon Young | - |
dc.date.issued | 2018-12-01 | - |
dc.identifier.issn | 1879-0909 | - |
dc.identifier.uri | https://aurora.ajou.ac.kr/handle/2018.oak/30358 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85053004261&origin=inward | - |
dc.description.abstract | Production of (Z)-11-(heptanoyloxy)undec-9-enoic acid from recinoleic acid was achieved by whole-cell biotransformation by Escherichia coli, utilizing crude glycerol as the sole carbon source. Whole-cell biotransformation resulted in ∼93% conversion of the substrate ricinoleic acid to (Z)-11-(heptanoyloxy)undec-9-enoic acid. We replaced the inducer-dependent promoter system (T7 and Rhm promotors) with a constitutive promoter system. This resulted in successful expression of ADH, FadL, and E6-BVMO, without costly inducer addition. Efficacy evaluation of the whole-cell biotransformation by inducer-free system by five different E. coli strains revealed that the highest product titer was accumulated in E. coli BW25113 strain. The engineered inducer-free system using crude glycerol as the sole carbon source showed competitive performance with induction systems. Optimized conditions resulted in the accumulation of 7.38 ± 0.42 mM (Z)-11-(heptanoyloxy)undec-9-enoic acid, and when 10 mM substrate was used as feed concentration, the product titer reached 2.35 g/L. The inducer-free construct with constitutive promoter system that this study established, which utilizes the waste by-product crude glycerol, will pave the way for the economic synthesis of many industrially important chemicals, like (Z)-11-(heptanoyloxy)undec-9-enoic acid. | - |
dc.description.sponsorship | The authors declare they have no conflict of interest. This work was supported by the Industrial Strategic Technology Development program (No. 10044604 , No. 20162220100260 ), funded by the Ministry of Trade, Industry & Energy (MI, Korea) . And this work was also supported by Next-Generation BioGreen21 Program (SSAC, No. PJ01312801) of RDA (Rural Development Administration) Korea and by the National Research Foundation of Korea (NRF) grant, funded by the Korea government (MEST) ( 2018R1D1A1B07046920 ). | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Inc. | - |
dc.subject.mesh | BVMO | - |
dc.subject.mesh | Constitutive promoters | - |
dc.subject.mesh | Crude glycerol | - |
dc.subject.mesh | Inducer-free expression | - |
dc.subject.mesh | Ricinoleic acid | - |
dc.subject.mesh | Bacterial Outer Membrane Proteins | - |
dc.subject.mesh | Biotransformation | - |
dc.subject.mesh | Carbon | - |
dc.subject.mesh | Escherichia coli | - |
dc.subject.mesh | Escherichia coli Proteins | - |
dc.subject.mesh | Genetic Engineering | - |
dc.subject.mesh | Glycerol | - |
dc.subject.mesh | Ricinoleic Acids | - |
dc.subject.mesh | Undecylenic Acids | - |
dc.title | Production of (Z)-11-(heptanoyloxy)undec-9-enoic acid from ricinoleic acid by utilizing crude glycerol as sole carbon source in engineered Escherichia coli expressing BVMO-ADH-FadL | - |
dc.type | Article | - |
dc.citation.endPage | 51 | - |
dc.citation.startPage | 45 | - |
dc.citation.title | Enzyme and Microbial Technology | - |
dc.citation.volume | 119 | - |
dc.identifier.bibliographicCitation | Enzyme and Microbial Technology, Vol.119, pp.45-51 | - |
dc.identifier.doi | 10.1016/j.enzmictec.2018.09.001 | - |
dc.identifier.pmid | 30243386 | - |
dc.identifier.scopusid | 2-s2.0-85053004261 | - |
dc.identifier.url | www.elsevier.com/locate/enzmictec | - |
dc.subject.keyword | (Z)-11-(heptanoyloxy)undec-9-enoic acid | - |
dc.subject.keyword | BVMO | - |
dc.subject.keyword | Constitutive promoter | - |
dc.subject.keyword | Crude glycerol | - |
dc.subject.keyword | Inducer-free expression | - |
dc.subject.keyword | Ricinoleic acid | - |
dc.type.other | Article | - |
dc.identifier.pissn | 0141-0229 | - |
dc.description.isoa | false | - |
dc.subject.subarea | Biotechnology | - |
dc.subject.subarea | Bioengineering | - |
dc.subject.subarea | Biochemistry | - |
dc.subject.subarea | Applied Microbiology and Biotechnology | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.