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Production of (Z)-11-(heptanoyloxy)undec-9-enoic acid from ricinoleic acid by utilizing crude glycerol as sole carbon source in engineered Escherichia coli expressing BVMO-ADH-FadL
  • Sudheer, Pamidimarri D.V.N. ;
  • Seo, Dahee ;
  • Kim, Eun Joo ;
  • Chauhan, Sushma ;
  • Chunawala, J. R. ;
  • Choi, Kwon Young
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dc.contributor.authorSudheer, Pamidimarri D.V.N.-
dc.contributor.authorSeo, Dahee-
dc.contributor.authorKim, Eun Joo-
dc.contributor.authorChauhan, Sushma-
dc.contributor.authorChunawala, J. R.-
dc.contributor.authorChoi, Kwon Young-
dc.date.issued2018-12-01-
dc.identifier.issn1879-0909-
dc.identifier.urihttps://aurora.ajou.ac.kr/handle/2018.oak/30358-
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85053004261&origin=inward-
dc.description.abstractProduction of (Z)-11-(heptanoyloxy)undec-9-enoic acid from recinoleic acid was achieved by whole-cell biotransformation by Escherichia coli, utilizing crude glycerol as the sole carbon source. Whole-cell biotransformation resulted in ∼93% conversion of the substrate ricinoleic acid to (Z)-11-(heptanoyloxy)undec-9-enoic acid. We replaced the inducer-dependent promoter system (T7 and Rhm promotors) with a constitutive promoter system. This resulted in successful expression of ADH, FadL, and E6-BVMO, without costly inducer addition. Efficacy evaluation of the whole-cell biotransformation by inducer-free system by five different E. coli strains revealed that the highest product titer was accumulated in E. coli BW25113 strain. The engineered inducer-free system using crude glycerol as the sole carbon source showed competitive performance with induction systems. Optimized conditions resulted in the accumulation of 7.38 ± 0.42 mM (Z)-11-(heptanoyloxy)undec-9-enoic acid, and when 10 mM substrate was used as feed concentration, the product titer reached 2.35 g/L. The inducer-free construct with constitutive promoter system that this study established, which utilizes the waste by-product crude glycerol, will pave the way for the economic synthesis of many industrially important chemicals, like (Z)-11-(heptanoyloxy)undec-9-enoic acid.-
dc.description.sponsorshipThe authors declare they have no conflict of interest. This work was supported by the Industrial Strategic Technology Development program (No. 10044604 , No. 20162220100260 ), funded by the Ministry of Trade, Industry & Energy (MI, Korea) . And this work was also supported by Next-Generation BioGreen21 Program (SSAC, No. PJ01312801) of RDA (Rural Development Administration) Korea and by the National Research Foundation of Korea (NRF) grant, funded by the Korea government (MEST) ( 2018R1D1A1B07046920 ).-
dc.language.isoeng-
dc.publisherElsevier Inc.-
dc.subject.meshBVMO-
dc.subject.meshConstitutive promoters-
dc.subject.meshCrude glycerol-
dc.subject.meshInducer-free expression-
dc.subject.meshRicinoleic acid-
dc.subject.meshBacterial Outer Membrane Proteins-
dc.subject.meshBiotransformation-
dc.subject.meshCarbon-
dc.subject.meshEscherichia coli-
dc.subject.meshEscherichia coli Proteins-
dc.subject.meshGenetic Engineering-
dc.subject.meshGlycerol-
dc.subject.meshRicinoleic Acids-
dc.subject.meshUndecylenic Acids-
dc.titleProduction of (Z)-11-(heptanoyloxy)undec-9-enoic acid from ricinoleic acid by utilizing crude glycerol as sole carbon source in engineered Escherichia coli expressing BVMO-ADH-FadL-
dc.typeArticle-
dc.citation.endPage51-
dc.citation.startPage45-
dc.citation.titleEnzyme and Microbial Technology-
dc.citation.volume119-
dc.identifier.bibliographicCitationEnzyme and Microbial Technology, Vol.119, pp.45-51-
dc.identifier.doi10.1016/j.enzmictec.2018.09.001-
dc.identifier.pmid30243386-
dc.identifier.scopusid2-s2.0-85053004261-
dc.identifier.urlwww.elsevier.com/locate/enzmictec-
dc.subject.keyword(Z)-11-(heptanoyloxy)undec-9-enoic acid-
dc.subject.keywordBVMO-
dc.subject.keywordConstitutive promoter-
dc.subject.keywordCrude glycerol-
dc.subject.keywordInducer-free expression-
dc.subject.keywordRicinoleic acid-
dc.type.otherArticle-
dc.identifier.pissn0141-0229-
dc.description.isoafalse-
dc.subject.subareaBiotechnology-
dc.subject.subareaBioengineering-
dc.subject.subareaBiochemistry-
dc.subject.subareaApplied Microbiology and Biotechnology-
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