이범진 이주현 2021-02 30770 https://aurora.ajou.ac.kr/handle/2018.oak/20057 학위논문(석사)--아주대학교 일반대학원 :약학과,2021. 2 1. Introduction 1 2. Materials and Methods 6 2.1. Materials 6 2.2. Preparation of LMN o/w emulsion 6 2.2.1. The effect of ratio of CTS and ES 100 7 2.2.2. Preparation of freeze fried LMN loaded microcapsules (LMN-MCs) 9 2.3. Characterization of LMN-MCs 9 2.3.1. Upright microscope 9 2.3.2. Measurement of Zeta potential 9 2.3.3. Evaluation of morphology of freeze dried LMN-MCs by SEM / EDS 10 2.3.4. DSC analysis 10 2.3.5. FT-IR analysis 10 2.3.6. Charge density analysis by Charge Analyzing System (CAS) 11 2.4. Measurement of Encapsulation efficiency (EE) and loading content (LC) 11 2.5. LMN release profile 12 2.6. Statistical analysis 12 3. Results and discussions 14 3.1. Optimization of formulation of LMN-MCs 14 3.2. Physicochemical properties of LMN-MCs 16 3.3. DSC analysis 21 3.4. FT-IR analysis 23 3.5. Charge density analysis by Charge Analyzing System (CAS) 25 3.6. LMN release profile 27 4. Conclusions 29 5. References 30 eng The Graduate School, Ajou University 아주대학교 논문은 저작권에 의해 보호받습니다. Preparation and characterization of interpolyelectrolyte complex microcapsules for controlled perfume release Thesis 아주대학교 일반대학원 일반대학원 약학과 2021. 2 Master I804:41038-000000030770 http://dcoll.ajou.ac.kr:9080/dcollection/common/orgView/000000030770 electrostatic interaction interpolyelectrolyte complexes microcapsule sustained release Although perfumes are used in various industries and daily life, they are limited in their use due to their high volatility and easy change due to exposure to the external environment. The purpose of this study was to design sustained release perfume with high encapsulation efficiency, using limonene (LMN) as a model perfume. Chitosan (CTS) has the properties of positive charge in aqueous solution, while Eudragit S100 (ES100) has the properties of the negative charge. Used the properties of these polymers, they formed interpolyelectrolyte complexes (IPECs) and improved LMN loaded efficiently and sustained release. The main method was formed to o/w emulsion using ultra-sonication, and microcapsule powder was manufactured using pH controlled and freeze-drying method. Encapsulation efficiency, loading content, and sustained release effect were evaluated using HPLC at different ratio of CTS and ES100 respectively. The tendency of release of LMN was measured by quantifying it at room temperature. And evaluated the physicochemical properties of LMN loaded capsules using DSC, FTIR, and CAS. The optimized capsule loaded with LMN showed 94% encapsulation efficiency and more than 120 hours of sustained effectiveness.