Research in the field of regenerative medicine, which replaces or restores the function of human damaged organs is advancing rapidly. These advances are fostering important innovations in the development of artificial organs. In recent years, three-dimensional (3D) bioprinting has emerged as a promising technology for regenerative medicine applications. Among various techniques, digital light process (DLP) 3D bioprinting stands out for its ability to precisely create high-resolution, structurally complex artificial organs. This review explores the types and usage trends of DLP printing equipment, bioinks, and photoinitiators. Building on this foundation, the applications of DLP bioprinting for creating precise microstructures of human organs and for regenerating tissue and organ models in regenerative medicine are examined. Finally, challenges and future perspectives regarding DLP-based bioprinting, particularly for precision printing applications in regenerative medicine, are discussed.
The liver is the largest internal organ and gland in the human body. Using a DLP-based 3D printer, Ma et al. encapsulated three types of cells\u2014human induced pluripotent stem cell (hiPSC)-derived hematopoietic progenitor cells (HPC), human umbilical vein endothelial cells (HUVEC), and adipose-derived stem cells\u2014to print numerous micro-hexagonal structures [122\u2013126]. By analyzing cell morphology, liver-specific gene expression levels, metabolic products, and cytochrome P450 induction, the authors determined that hiPSC-HPCs in the printed liver model closely resembled human liver cells. The results indicate that the microstructure and supporting cells promote the maturation and maintenance of hiPSC-HPC functions.The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Moon Suk Kim reports financial support was provided by Ajou University. Moon Suk kim reports a relationship with Ajou University that includes: employment. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.This study was supported by the National Research Foundation of Korea (NRF) grants, Creative Materials Discovery Program (2019M3D1A1078938) and Future Promising Fusion Technology Pioneer Program (RS-2024-00458419).This study was supported by the National Research Foundation of Korea (NRF) grants, Creative Materials Discovery Program (2019M3D1A1078938) and STEAM Program (RS-2024-00458419).
