Prostate cancer (PCa) is the most prevalent cancer worldwide, with a high mortality rate. The early and accurate detection of PCa is critical in reducing mortality and saving lives. Timely diagnosis can improve the chances of successful treatment using advanced technologies. In recent years, nanomaterial-based electrochemical sensing strategies have been adopted in clinical diagnosis, as they allow sensitive early-biomarker detections to be converged with a cost-effective electronic readout system. Herein, we present a flexible electrochemical immunosensor platform for detecting interleukin-6 (IL-6) based on an Au-integrated flexible carbon fiber (Au/CF) electrode prepared via electrodeposition and chemically modified to capture IL-6 antibodies. Several techniques are used to analyze the prepared Au/CF composite electrodes to confirm their morphology, structure, and elemental composition. Under optimum conditions, the fabricated immunosensor exhibits a wide linear dynamic ranging from 1 fg/mL to 1 μg/mL and a low detection limit of 0.056 fg/mL, with a sensitivity of 62.17 μA/(fg mL−1). The proposed fiber-based immunosensor is used to quantify the concentration of IL-6 in serum samples from clinical PCa patients (T3b and T4 stages), and the results are validated using the commercial Meso Scale Diagnostics (MSD) V-Plex method. The acceptable results yielded by the proposed immunosensor indicate that it can serve as a new platform to realize highly sensitive and cost-effective diagnostic strategies for the early diagnosis of PCa.
This study was supported by the Brain Pool program funded by the Ministry of Science and ICT through the National Research Foundation of Korea (NRF-2020H1D3A1A04077592), the National R&D Program for Cancer Control (HA17C0039), the new faculty research fund of Ajou University and a grant from the Patient-Centered Clinical Research Coordinating Center (HI19C0481 and HC19C0164). The biospecimens and data used in this study were provided by the Biobank of the Seoul National University Hospital, which is a member of the Korea Biobank Network (KBN4_A03). We thank Ja Hyeon Ku and Cheol Kwak at the Department of Urology at Seoul National University Hospital for providing patient samples.This study was supported by the Brain Pool program funded by the Ministry of Science and ICT through the National Research Foundation of Korea ( NRF-2020H1D3A1A04077592 ), the National R&D Program for Cancer Control ( HA17C0039 ), the new faculty research fund of Ajou University and a grant from the Patient-Centered Clinical Research Coordinating Center ( HI19C0481 and HC19C0164 ). The biospecimens and data used in this study were provided by the Biobank of the Seoul National University Hospital, which is a member of the Korea Biobank Network (KBN4_A03). We thank Ja Hyeon Ku and Cheol Kwak at the Department of Urology at Seoul National University Hospital for providing patient samples.
