Rhodococcus sp. YHY01 was studied to utilize various lignin derived aromatic compounds. It was able to utilize p-coumaric acid, cresol, and 2,6 dimethoxyphenol and resulted in biomass production i.e. 0.38 g dcw/L, 0.25 g dcw/L and 0.1 g dcw/L, and lipid accumulation i.e. 49%, 40%, 30%, respectively. The half maximal inhibitory concentration (IC50) value for p-coumaric acid (13.4 mM), cresol (7.9 mM), and 2,6 dimethoxyphenol (3.4 mM) was analyzed. Dimethyl sulfoxide (DMSO) solubilized barley straw lignin fraction was used as a carbon source for Rhodococcus sp. YHY01 and resulted in 0.130 g dcw/L with 39% w/w lipid accumulation. Major fatty acids were palmitic acid (C16:0) 51.87%, palmitoleic acid (C16:l) 14.90%, and oleic acid (C18:1) 13.76%, respectively. Properties of biodiesel produced from barley straw lignin were as iodine value (IV) 27.25, cetane number (CN) 65.57, cold filter plugging point (CFPP) 14.36, viscosity (υ) 3.81, and density (ρ) 0.86.
The authors would like to acknowledge the KU Research Professor Program of Konkuk University, Seoul, South Korea. This work was supported by Research Program to solve social issues of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017M3A9E4077234) and National Research Foundation of Korea (NRF) (NRF-2015M1A5A1037196, 2017R1D1A1B03030766 and NRF-2019M3E6A1065160). In addition, this work was also supported by polar academic program (PAP, PE18900). This work was also supported by Next-Generation BioGreen21 Program (SSAC, PJ01312801), Rural Development Administration. The consulting service of the Microbial Carbohydrate Resource Bank (MCRB, Seoul, South Korea) is greatly appreciated.The authors would like to acknowledge the KU Research Professor Program of Konkuk University , Seoul, South Korea. This work was supported by Research Program to solve social issues of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2017M3A9E4077234) and National Research Foundation of Korea (NRF) ( NRF-2015M1A5A1037196 , 2017R1D1A1B03030766 and NRF-2019M3E6A1065160 ). In addition, this work was also supported by polar academic program (PAP, PE18900). This work was also supported by Next-Generation BioGreen21 Program (SSAC, PJ01312801), Rural Development Administration. The consulting service of the Microbial Carbohydrate Resource Bank (MCRB, Seoul, South Korea) is greatly appreciated.
