Hypochlorous acid (HOCl) is one important member of reactive oxygen species (ROS) familyresponsible for various human diseases. Although many contributions have been made to develop fluorescent probes for sensing HOCl, many of them suffer small Stokes shifts, sophisticated synthetic procedure, and slow reaction rate. In the current study, we designed and synthesized an ESIPT based fluorescence probe with diaminomeleonitrile (DMN) as reactive site. The probe can detect HOCl with high selectivity and sensitivity. It also has large Stokes shift and rapid reaction rate. Moreover, it can be utilized to monitor exogenous and endogenous HOCl in living cells and tissues via two-photon microscopy.
This study was supported by grants of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) ( 2012R1A3A2048814 to J. Y.; 2016R1E1A1A02920873 to H. M. K) Mass spectral data were ob-tained from the Korea Basic Science Institute (Daegu) using a Jeol JMS 700 high-resolution mass spectrometer.
