A novel type strain, Planococcus faecalis AJ003T, isolated from the feces of Antarctic penguins, synthesizes a rare C30 carotenoid, glycosyl-4,4ʹ-diaponeurosporen-4ʹ-ol-4-oic acid. The complete genome of P. faecalis AJ003T comprises a single circular chromosome (3,495,892 bp; 40.9% G + C content). Annotation analysis has revealed 3511 coding DNA sequences and 99 RNAs; seven genes associated with the MEP pathway and five genes involved in the carotenoid pathway have been identified. The functionality and complementation of 4,4ʹ-diapophytoene synthase (CrtM) and two copies of heterologous 4,4ʹ-diapophytoene desaturase (CrtN) involved in carotenoid biosynthesis were analyzed in Escherichia coli.
This work was supported by a National Research Foundation of Korea (NRF) grant funded by the government of South Korea ( 2012M1A2A2671691 ), and by the Technology Innovation Program (No. 10051375) funded by the Ministry of Trade, Industry & Energy (MI, Korea) .
