The emerging field of nanobiotechnology offers the potential for the development of exquisitely sensitive diagnostics and organ/tumor-targeted therapies [1]. Over the past few decades, there has been considerable interest in developing nanoparticles as effective drug delivery devices [1]. MNPs@SiO2(RITC) are expected to have a wide range of applications in the future because they are easy to synthesize and have good biocompatibility by coated with SiO2 [2]. However, there are limits in assessment of potential toxicity on the conventional method.
Here, I report the analysis for more detailed assessment of nanotoxicity with multidisciplinary method which is combined with conventional method. In chapter 1, general introduction was described about nanoparticle exposure, toxicity of nanoparticle, and nanoparticle used in my study in assessment of toxicity. In chapter 2, I assessed the changes in polyamine profile by silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate dye (MNPs@SiO2(RITC)) in human neuroblastoma SH-SY5Y cells. In chapter 3, I investigated the characteristics and in vitro cytotoxicity of human bone marrow-derived mesenchymal stem cells (hBM-MSC) labeled with MNPs@SiO2(RITC). I addressed this issue in the present study by investigating deformation, membrane fluidity, cytoskeletal change, the expression of genes related to lipid peroxidation and abnormal cytoskeleton, and migratory activity of hBM-MSC. In chapter 4, I assessed the microglial MNPs@SiO2(RITC)-induced activation by morphological analysis, surface marker protein analysis, transcriptome analysis, intracellular amino acid profiles, and secreted serine family amino acid and neuronal toxicity in co-culture system with neuronal cells, SH-SY5Y, primary rat cortical and dopaminergic neurons.
In conclusion, I studied nanotoxicity in neuronal cell line, stem cells, and primary neuronal cells with combinatorial analysis. That is, it should be taken into consideration in potential chronic toxicity of nanoparticle in usage for human.
