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쥐에서 미세구슬주입술을 이용한 만성 고안압증 모델; 폴리우레탄과 폴리메틸메타크릴레이트, 폴리스티렌재질의 비교
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Advisor
안재홍
Affiliation
아주대학교 일반대학원
Department
일반대학원 의학과
Publication Year
2015-08
Publisher
The Graduate School, Ajou University
Keyword
Chronic ocular hypertensionMicrobeadAxonRetinal ganglion cellIntraocular pressurePolyurethane
Description
학위논문(박사)--아주대학교 일반대학원 :의학과,2015. 8
Alternative Abstract
Purpose: To establish and assess an ocular hypertensive rat model using intracameral injection with various microbeads of different sizes and materials. Methods: Chronic elevation of intraocular pressure (IOP) was induced by injection of various microbeads into the anterior chamber of Sprague-Dawley rat eyes. We compared the IOPs induced by the injection of different microbeads [7- and 17-µm polyurethane (PU), 7- and 15-µm polymethylmethacrylate (PMMA), and 15-µm polystyrene (PS)] and selected the appropriate microbeads for a chronic ocular hypertensive model in terms of IOP elevation and adverse events. IOP changes were observed for 4 weeks after microbead injections. Axonal degeneration was assessed with transmission electron microscopic photographs and RGC loss was assessed with retrograde labeling. Results: Sixty-nine rats were included. Three days after a single injection of microbeads, IOPs were increased by 24.0% by 7-µm PU microbeads, 101.8% by 17-µm PU microbeads, 56.6% by 7-µm PMMA microbeads, 22.0% by 15-µm PMMA microbeads, and 34.7% by 15- µm PS microbeads. 17-µm PU microbeads produced constant IOP elevation with good reproducibility (standard deviation of < 6.6 mmHg). Sustained IOP elevation by two injections of 17-µm PU microbeads resulted in a 42% axon loss and 36.5% RGC loss (p<0.05, Mann-Whitney U test). Conclusions: PU microbead injections offer an applicable and versatile model for a chronic ocular hypertensive model in rats. Among several biomaterials, PU microbeads produced a more stable IOP elevation without adverse events.
Language
eng
URI
https://dspace.ajou.ac.kr/handle/2018.oak/18758
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Type
Thesis
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